Download e-book for iPad: Advances in Immunology, Vol. 31 by Henry G. Kunkel (ed.), Frank J. Dixon (ed.)

By Henry G. Kunkel (ed.), Frank J. Dixon (ed.)

ISBN-10: 0120224313

ISBN-13: 9780120224319

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Additional info for Advances in Immunology, Vol. 31

Example text

The results suggest that macrophageT cell contact induced by nonantigenic stimuli may result in interactions with the Ia antigens that generate the “signals” required for macrophage and T-cell stimulation. c. INTERACTIONS WITH T AND B CELLS IN ANTIBODY FORMATION The requirements for phagocytes in the immune response to thymus-dependent antigens have been examined extensively in a number of experimental systems. These systems vary from in uiuo cell transfer using live macrophages with bound antigen, to cell culture assays, to approaches combining both in vioo and cell culture techniques.

This finding contrasts with the results showing requirements for Ia-positive macrophages-whether the embryo cells bore Ia or contained macrophages was not studied. The issue of the requirement for an Ia-bearing cell was analyzed in a different way by Ahman et al. , 1980a). Such treated cells, however, could proliferate when a preparation of Iabearing spleen accessory cells was added to the culture. The accessory MACROPHAGES IN ANTIGENIC STIMULATION 19 cells were adherent to glass, lacked T-cell determinants, and contained two I-region determinants, one mapped to I-A, the other mapped from I-B to I-EIC.

The latter method has proved to be a simple, fast, and reproducible one for eliminating accessory cells. 3 . Studies with 2-Mereaptoethanol This reducing agent is a promoter of lymphocyte function and has been discussed as a possible substitute for macrophages in culture. Although the effects of thiols have been examined in a number of situations, some of which we have alluded to before, their actions have been particularly emphasized and discussed in the context of antibody formation in uitro.

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Advances in Immunology, Vol. 31 by Henry G. Kunkel (ed.), Frank J. Dixon (ed.)

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