Get Advances in Applied Microbiology, Vol. 35 PDF

By Saul L. Neidleman (ed.), Allen I. Laskin (ed.)

ISBN-10: 012002635X

ISBN-13: 9780120026357

Meant for researchers in utilized microbiology and environmental engineers, this booklet covers such issues as environmental overview of biotechnological tactics and microbial differences of haloaromatic and haloaliphatic compounds.

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The duration of exposure to the X radiation was at least 30 minutes in a range of 1-4 hours. The colony was then cultured in plane culture in a shallow tray at 33°C for 24 hours using solid medium of the same composition. One mutant with the capacity to produce a-amylase and another for production of protease were recovered. The X-ray treatment thus resulted in a mutant that produces a-amylase, without coproduction of protease, if a suitable medium such as one based on wheat was employed. The other mutant, when grown on cornmeal-based medium, produced protease.

Megaterium 16M compared to -33,000 units/g dry bacterial bran in the SSF process (Ramesh and Lonsane, 1987a). Data on the comparative yield of the enzyme by LSF and SSF techniques are also available. Based on the yield per gram of dry bran, the enzyme production by B. These results establish the formation of higher titers of enzyme in the SSF technique as compared to LSF and SmF processes. 0. RECOVERYOF ENZYME Methodology, developed over a long period of time and employed presently for extraction of fungal a-amylase from moldy substrates produced in the SSF technique, will also be most appropriate and effective for the extraction of bacterial a-amylase from bacterial bran.

B. , 1979; Underkofler, 1976). The bacterial cultures reported for the production of a-amylases in the SSF process are also limited to the genus Bacillus (Table 11). Beckord et al. (1945) studied 16 starch-hydrolyzing bacterial cultures for their ability to produce the enzyme in the SSF technique and reported that 11 cultures were able to produce a-amylases in varying amounts. Among these, the capability to produce high titers of the enzyme was confined to three species (B. subtilis, B. mesentericus, and B.

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Advances in Applied Microbiology, Vol. 35 by Saul L. Neidleman (ed.), Allen I. Laskin (ed.)

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